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Beta-glucanase can hydrolyze beta-glucan in plant feed ingredients to enhance utilization of nutrients in animal feed, such as energy, crude protein, amino acids, etc.
Definition of Activity
One unit (U) of glucanase is defined as the quantity of enzyme, which liberates 1μmol reducing sugar per minute from 4mg/mL β-glucan solution at 37℃, pH5.5.
Anti-nutrient Effects of Beta-glucan
Beta-glucan, as a kind of non-starch polysaccharide (NSP), is one of the most important anti-nutritional factors in wheat and feed meal stuffs (table1).
Table 1 Content of β-glucan in common plant feed stuffs
Feed stuffs | corn | wheat | barley | Sorghum | rice | Wheat bran | middling | rice bran | Soybean meal | Cottonseed meal | Rapeseed meal | Sunflower seed meal |
content(%) | minim | 0.7 | 3.7 | 0.2 | 0.1 | 0.3 | 1.7 | minimum | 5.8 | 4.5 | 5.1 | 7.8 |
1. The cell wall contains high level of Beta-glucan. Its "package" effect can reduce the diffusion speed of nutrients and the probability that nutrients contacts with digestive enzymes, thereby reducing the utilization of nutrients.
2. The dissolution of Beta-glucan increases the viscosity of the chyme and enlarges the volume of intestinal tract after water-swelling , thus reducing the diffusion rate of enzyme and substrate, hindering digested nutrients to approach the surface of small intestine, ultimately leading to lower nutrient digestibility.
3. Combine Beta-glucan with glycoprotein of intestinal mucosal epithelial cells to form a thickened and fixed water layer, thus limiting the speed of its absorption.
4. Beta-glucan can be combined with some digestive enzymes and bile salts, all of which are the physiological substances to assist digestion.
Product Characteristics
Graph1. Glucanase Temperature Curve
Graph2. Glucanase pH Curve
Graph3. Glucanase Stability Curve
Graph4. Glucanase Thermostability Curve (Under 85% humidity for 5min)
1. High glucan specificity, especially having a significant degradation of glucan in corn, wheat and other gramineous feedstuffs.
2. Advanced liquid submerged fermentation production technology, unique pose processing techniques, good stability and suitable for different feed.
3. Long effect time and broad pH range of activity help it play a role in the whole digestive tract.
4. Little affected by the metal ions and protease.
Table1. The Effect of Metal Ion on the activity of Glucanase
Metal Ion | Concentration C/mol.L-1 | Relative activity/% | Evaluation |
Fe2+ Ca2+ Mn2+ Cu2+ Zn2+ Comparison | 10-3 10-3 10-3 10-3 10-3 / | 87.53 96.31 92.78 107.52 101.40 100 | - - 0 0 0 0 |
Note: + refers to activation; - refers to inhibition; 0 refers to no obvious impact (the effect on enzyme activity is within 10%)
Product Functions
1. High efficiency of degradation of beta glucan, increasing the nutrient digestibility of plant materials, especially wheat and feed meals and improving the production performance of livestock and poultry;
2. Reducing viscosity of chime, improving the nutrients digestibility and usage;
3. Breaking down the cell wall, thus making the grain crude protein, fat, carbohydrates in the cells more easily to be absorbed;
4. Reducing the harmful bacteria breeding, improving the intestinal morphology, make the intestinal tract easier to absorb the nutrients;
5. More non-conventional raw materials could be used to lower feed costs while maintaining the production performance;
6. Reducing the phenomenon “anal paste” of livestock and poultry, in this way, feces can be cleaned easily, thus reducing the emission of nitrogen in manure.
Specification and Usage
Types | Activity | Usage |
powder | 10000U/g, 20000U/g | Usually used with other single enzymes (xylanase, etc.) together. Adding 50g (mL) with activity 10000U/g (mL) glucanase per metric tone. |
granule | ||
liquid | 10000U/mL, 20000U/mL |
Package and Storage
Packaging: Solid: 25 kg / bag; Liquid: 30 kg/plastic barrel
Store under sealed, room temperature (less than 25℃)condition for 12 months in original package(liquid:6 months)
Avoid to be exposed to the sun, rain, high temperature and high humidity
Technical Service
Offer glucanase usage formula, enzyme activity test and related product technical service to ensure the using result
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