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Beta Glucanase

Beta-glucanase can hydrolyze beta-glucan in plant feed ingredients to enhance utilization of nutrients in animal feed, such as energy, crude protein, amino acids, etc.
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Product Description

Beta-glucanase can hydrolyze beta-glucan in plant feed ingredients to enhance utilization of nutrients in animal feed, such as energy, crude protein, amino acids, etc.


beta glucanase


Definition of Activity

One unit (U) of glucanase is defined as the quantity of enzyme, which liberates 1μmol reducing sugar per minute from 4mg/mL β-glucan solution at 37℃, pH5.5.


Anti-nutrient Effects of Beta-glucan

Beta-glucan, as a kind of non-starch polysaccharide (NSP), is one of the most important anti-nutritional factors in wheat and feed meal stuffs (table1).


Table 1 Content of β-glucan in common plant feed stuffs

Feed stuffs

corn

wheat

barley

Sorghum

rice

Wheat bran

middling

rice bran

Soybean meal

Cottonseed meal

Rapeseed meal

Sunflower seed

meal

content(%)

minim

0.7

3.7

0.2

0.1

0.3

1.7

minimum

5.8

4.5

5.1

7.8


1. The cell wall contains high level of Beta-glucan. Its "package" effect can reduce the diffusion speed of nutrients and the probability that nutrients contacts with digestive enzymes, thereby reducing the utilization of nutrients.

2. The dissolution of Beta-glucan increases the viscosity of the chyme and enlarges the volume of intestinal tract after water-swelling , thus reducing the diffusion rate of enzyme and substrate, hindering digested nutrients to approach the surface of small intestine, ultimately leading to lower nutrient digestibility.

3. Combine Beta-glucan with glycoprotein of intestinal mucosal epithelial cells to form a thickened and fixed water layer, thus limiting the speed of its absorption.

4. Beta-glucan can be combined with some digestive enzymes and bile salts, all of which are the physiological substances to assist digestion.


Product Characteristics

图片1

Graph1. Glucanase Temperature Curve


图片2

Graph2. Glucanase pH Curve


图片3

Graph3. Glucanase Stability Curve


图片4

Graph4. Glucanase Thermostability Curve (Under 85% humidity for 5min)


1. High glucan specificity, especially having a significant degradation of glucan in corn, wheat and other gramineous feedstuffs.

2. Advanced liquid submerged fermentation production technology, unique pose processing techniques, good stability and suitable for different feed.

3. Long effect time and broad pH range of activity help it play a role in the whole digestive tract.

4. Little affected by the metal ions and protease.


Table1. The Effect of Metal Ion on the activity of Glucanase

Metal Ion

Concentration C/mol.L-1

Relative activity/%

Evaluation

Fe2+

Ca2+

Mn2+

Cu2+

Zn2+

Comparison

10-3

10-3

10-3

10-3

10-3

/

87.53

96.31

92.78

107.52

101.40

100

-

-

0

0

0

0

Note: + refers to activation; - refers to inhibition; 0 refers to no obvious impact (the effect on enzyme activity is within 10%)


Product Functions

1. High efficiency of degradation of beta glucan, increasing the nutrient digestibility of plant materials, especially wheat and feed meals and improving the production performance of livestock and poultry;

2. Reducing viscosity of chime, improving the nutrients digestibility and usage;

3. Breaking down the cell wall, thus making the grain crude protein, fat, carbohydrates in the cells more easily to be absorbed;

4. Reducing the harmful bacteria breeding, improving the intestinal morphology, make the intestinal tract easier to absorb the nutrients;

5. More non-conventional raw materials could be used to lower feed costs while maintaining the production performance;

6. Reducing the phenomenon “anal paste” of livestock and poultry, in this way, feces can be cleaned easily, thus reducing the emission of nitrogen in manure.


Specification and Usage

Types

Activity

Usage

powder

10000U/g, 20000U/g

Usually used with other single enzymes (xylanase, etc.) together. Adding 50g (mL) with activity 10000U/g (mL) glucanase per metric tone.

granule

liquid

10000U/mL, 20000U/mL


Package and Storage

Packaging: Solid: 25 kg / bag; Liquid: 30 kg/plastic barrel

Store under sealed, room temperature (less than 25℃)condition for 12 months in original package(liquid:6 months)

Avoid to be exposed to the sun, rain, high temperature and high humidity


Technical Service

Offer glucanase usage formula, enzyme activity test and related product technical service to ensure the using result






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Shanghai Rindazhi Biochemical Technology Co., Ltd. supplies qualified chemical products including industrial enzymes, food grade enzymes, food additives, health supplement products, vitamins, flavors, etc. Rindazhi is sold based on its brother and sister company Co-Suppliers Limited which has producing bases, research labs, and technical teams in different part of China.
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