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Alpha-galactosidase is an enzyme used to hydrolyze or break Alpha-1, 6-glycosidic bonds in galactosyl oligosaccharides (Alpha-galactosides), liberating simpler, more usable sugars and eliminating its anti-nutrient effect. It can be used as food additive or healthe supplement.
Definition activity
One unit (U) of galactosidase is defined as the quantity of enzyme, which liberates 1μmol P-nitrophenol in one min at 37℃ and pH5.0
Mechanisms
Alpha-galactoside (such as raffinose and stachyose) is an anti-nutrient factor present in some plant seeds. Its concentration is the highest in bean seeds and lowest in grain seed.
Content of Alpha-galactoside in common plant feed stuff
Plant seed | Raffinose（%） | Stachyose（%） | Total |
Soy bean | 1.21 | 4.22 | 5.43 |
Cottonseed | 6.91 | 2.36 | 9.27 |
Peanut | 0.33 | 0.99 | 1.32 |
Sunflower seed | 3.09 | 0.14 | 3.23 |
Wheat | 0.70 | Tr | 0.70 |
Barley | 0.71 | Tr | 0.71 |
Corn | 0.26 | Tr | 0.26 |
Note: Tr refers to trace in quantity.
Alpha-galactosidase can digest Alpha-galactosides such as raffinose and stachyose. It can also synergize with mannanase to hydrolyze galactomannans contained in bean gums.
Product characteristic
Graph2 Alpha-galactosidase Temperature Curve
Graph3 Alpha-galactosidase pH Curve
Graph4 Alpha-galactosidase Thermostability Curve
Graph5 Alpha-galactosidase Stability Curve
Table 2 The effect of metal ion on the enzyme activity of alpha-galactosidase
Metal Ion | Concentration/mol.L-1 | Relative enzyme activity/% | comment |
Fe2+ Ba2+ Mg2+ Ca2+ Mn2+ Cu2+ Zn2+ Control | 10-3 10-3 10-3 10-3 10-3 10-3 10-3 / | 97.31 89.37 117.24 120.18 82.46 96.06 95.43 100.00 | 0 - + + - 0 0 0 |
Note: + stands for positive effect, - stands for negative effect; 0 stands for unobvious effect( within 10%)
Specification and Usage
Specification | Enzyme Activity | Usage |
Powder | 3000-10000U/g； | Recommend using alpha-galactosidase in conjunction with mannanase. |
Package and Storage
Solid products: 25kg/Kraft-bag or 20kg/Barrel;
Store in original package under sealed, room temperature condition for 12 months to solid form, 6 months for liquid form.
Avoid to be exposed to the sun, rain, high temperature and high humidity.
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