Cellulase

Cellulase was produced by Trichodermaspecies using modern genetic engineering technology, microbial fermentation technology, and advanced post-processing techniques. It is composed of multiple ingredients and can degrade cellulose in fibers to create simple sugars efficiently, thus improving the utilization of raw ingredients. It is widely used in manufacturing of feed, textile, fuel ethanol, alcohol, Chinese medicine extraction, food, fruit juice-processing, etc.

Mechanism

Cellulose is a high-polysaccharides made of glucopyranose by β-1, 4-glycosidic bond. Cellulase system consists of three major components: Endoglucanases (endo-1, 4-β-D-glucanases; C1; EG, EC 3.2.1.4), cellobiohydrolases (exo-1, 4-β-D-glucanases; CX; CBH, EC 3.2.1.91), and β-glucosidases (1, 4-β-D-glucosidase; βG; BGL, EC 3.2.1.21). EG acts on insoluble cellulose surface, breaks internal bonds to disrupt the crystalline structure of cellulose and expose individual cellulose polysaccharide chains, and makes cellulose chains hydration easily. CBH cleaves 2-4 units from the ends of the exposed chains produced by EG, resulting in the tetrasaccharides or disaccharide such as cellobiose. There are two main types of CBH- one type working processively from the reducing end, and the other type working processively from the non-reducing end of cellulose. βG hydrolyses the CBH product into individual monosaccharides. Through the synergistic action of above enzyme system, cellulose can be efficiently hydrolyzed to glucose.

Specification

Definition

1 unit of CMCase equals to the amount of enzyme, which hydrolyzes CMC-Na to get 1 μg of reducing sugar (in glucose) in 1 min. at 50℃ and pH4.8.

Product Functions

1. Feed Industry: Degrading cellulose to cello-oligosaccharide specifically, decreasing the chyme mucosity. Breaking down the structure of cell walls and releasing the nutrients inside, increasing the diffusion rate of endogenous digestive enzymes and enhancing the nutrients digestibility. Balancing intestinal flora to enhance animal immunity and reduce diarrhea ratio. Improving the animal production performance to increase customer profits.

2. Fabric and Textiles: Improving the finish, sheen, flexibility, air-permeability, insulation and softness of the textile fabrics, developing permanent resistance of fabric to lint, fuzz and pill buildups and easing further treatments and dye processes.

3. Breweries and Wineries: Breaking down plant cell walls and membranes, boosting the release of starch, and in turn, the output of alcohol.

4. Food, Chinese medicine, etc.: Improving the effectiveness, absorption and nutritional effects of product.

Product features

1. Full –scale enzyme profile (EG, CBH and βG) ensures the efficient degradation of cellulose.

2. Optimal genetic engineering strain, advanced production technology of liquid submerged fermentation and unique post-processing lead high cellulase activity and good stability.

Broad enzyme profile, high enzyme activity and good thermostability help it more suitable for varieties of processing.

Usage

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